Coding

Part:BBa_K1808008:Design

Designed by: Remsha Afzal   Group: iGEM15_TrinityCollegeDublin   (2015-09-16)


Amorpha-4,11-diene synthase His tagged


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1503
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 935
    Illegal XhoI site found at 685
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1195
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is codon optimised for BioBrick 3A assembly and expression in E. coli cells as well as protein purification.


Source

Artemisia annua

References